Huntsville is the most populous city in the U.S. state of Alabama. The population of the city is estimated to be 241,114 in 2025, making it the 102nd-most populous city in the U.S. The Huntsville metropolitan area had an estimated 514,465 residents and is the second-most populous metro area in the state, after Birmingham. Huntsville is the seat of Madison County, with portions extending into Limestone County and Morgan County.